NEU259: Introduction to Light and Electron Microscopy (Spring 2009)
Instructors:
Maryann Martone, Ph. D.; mmartone@ucsd.edu, X20745
Gina Sosinsky, Ph. D.; gsosinsky@ucsd.edu, X40128
Mark Ellisman, Ph. D.; mellisman@ucsd.edu, X41392
Time and Location: Tuesday/Thursday 10:00 - 11:00 am, 1000 BSB Conference Room
Thursday demo: 11:00 - 12:30, Location to be announced
Syllabus: (document download)
Readings: Readings will be assigned by instructor and will be available with our administrative assistants for the NCMIR facility. A web course on electron microscopy can be found at http://em-outreach.ucsd.edu.
Our course websites can be accessed at: http://ncmir.ucsd.edu/training/ucsd_courses/index.shtm
No. |
Date |
Topic |
Lecturer |
Demo |
1 |
31-Mar |
Intro to Course / Intro to Optics I |
Maryann Martone/ James Bouwer |
|
2 |
2-Apr |
Intro to Optics II |
James Bouwer |
LM - MM/TD |
3 |
7-Apr |
Confocal Microscopy /Fluorescence |
Tom Deerinck |
|
4 |
9-Apr NOTE: |
Transmission Electron Microscopy LECTURE ROOM CHANGE THIS DATE ONLY: |
Tom Deerinck LEICHTAG 2A05 |
TEM – TD |
5 |
14-Apr |
Multiphoton Microscopy |
James Bouwer |
|
6 |
16-Apr |
Optical probes for live imaging |
Guido Gaietta |
NCMIR Tour-ME |
7 |
21-Apr |
Immunocytochemistry for LM and EM |
Maryann Martone |
|
8 |
23-Apr |
Specimen Preparation |
Maryann Martone |
Confocal – Hiro Hakozaki |
9 |
28-Apr |
In Situ Hybridization |
Armin Blesch |
|
10 |
30-Apr |
Midterm |
|
No Demo |
11 |
5-May |
Advance Light Microscopy Techniques (e.g. TIRF, near field) |
Hiro Hakozaki |
|
12 |
7-May |
Digital Imaging |
James Bouwer |
TBA |
13 |
12-May |
Image Processing/Analysis Fundamentals |
Gina Sosinsky |
|
14 |
14-May |
Deconvolution Microscopy |
James Bouwer |
Adobe Photoschop-TD/Deconvolution Demo ImageJ (Hakozaki) |
15 |
19--May |
Meso-scale imaging techniques |
Mark Ellisman |
|
16 |
21-May |
Electron Tomography |
Guy Perkins |
Tomography |
17 |
26-May |
Molecular Microscopy: Specimen Prep |
Norm Olson |
|
18 |
28-May |
Molecular Microscopy: 3D Recon Methods |
Tim Baker |
CryoEM (Olson) |
*19 |
2-June |
Scanning Probe Microscopies |
Gina Sosinsky |
SEM demo (tentative) |
20 |
4-Jun |
OPEN |
|
|
** Finals Week |
9-Jun |
Future of Microscopy & Student Presentations |
All instructors |
|
* Written assignment "My thesis project and how microscopy cna contribute to my thesis." (1 page limit is for the body of writing. Use Arial font, 11 pt, 1 inch margins (2.5 cm) relative to 8.5 x 11 inch paper format. Line spacing should be 6 lines/inch (use single spacing with Microsoft Word).
**Final examination is take-home and due back by June 11th.
